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mouse biotinylated anti human il 10r polyclonal ab  (R&D Systems)


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    Structured Review

    R&D Systems mouse biotinylated anti human il 10r polyclonal ab
    FIGURE 1. Immunocytochemical staining of intracellular <t>IL-10R</t> in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.
    Mouse Biotinylated Anti Human Il 10r Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+biotinylated+anti+human+il+10r+polyclonal+ab/Human+IL-22+Biotinylated+Antibody/pm11290804-52-67-73
    Average 93 stars, based on 67 article reviews
    mouse biotinylated anti human il 10r polyclonal ab - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators."

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.166.8.5201

    FIGURE 1. Immunocytochemical staining of intracellular IL-10R in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.
    Figure Legend Snippet: FIGURE 1. Immunocytochemical staining of intracellular IL-10R in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.

    Techniques Used: Staining, Negative Control, Control, Light Microscopy

    FIGURE 2. Immunoblotting of IL-10R in human neutrophils. A total cavitas, specific and azurophilic granules, membranes, and cytosol were prepared as described in Materials and Methods. Western blots were probed with a rabbit anti-IL-10R polyclonal Ab. A positive control (mono- nuclear cells) was loaded and blotted in parallel. The molecular masses of protein standards are indicated in kilodaltons.
    Figure Legend Snippet: FIGURE 2. Immunoblotting of IL-10R in human neutrophils. A total cavitas, specific and azurophilic granules, membranes, and cytosol were prepared as described in Materials and Methods. Western blots were probed with a rabbit anti-IL-10R polyclonal Ab. A positive control (mono- nuclear cells) was loaded and blotted in parallel. The molecular masses of protein standards are indicated in kilodaltons.

    Techniques Used: Western Blot, Positive Control

    FIGURE 3. Translocation of IL-10R from specific granules to the plasma membrane in PMA-stimulated PMN. PMN were incubated for 10 min in the presence or absence of PMA (100 ng/ml) at 37°C. Specific granules and membranes were prepared as described in Materials and Methods. West- ern blots were probed with a rabbit anti-IL-10R polyclonal Ab.
    Figure Legend Snippet: FIGURE 3. Translocation of IL-10R from specific granules to the plasma membrane in PMA-stimulated PMN. PMN were incubated for 10 min in the presence or absence of PMA (100 ng/ml) at 37°C. Specific granules and membranes were prepared as described in Materials and Methods. West- ern blots were probed with a rabbit anti-IL-10R polyclonal Ab.

    Techniques Used: Translocation Assay, Clinical Proteomics, Membrane, Incubation

    Related Articles

    Recombinant:

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
    Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland). ..

    Produced:

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
    Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland). ..

    Protease Inhibitor:

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
    Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland). ..

    Purification:

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
    Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland). ..



    Similar Products

    93
    R&D Systems mouse biotinylated anti human il 10r polyclonal ab
    FIGURE 1. Immunocytochemical staining of intracellular <t>IL-10R</t> in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.
    Mouse Biotinylated Anti Human Il 10r Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+biotinylated+anti+human+il+10r+polyclonal+ab/Human+IL-22+Biotinylated+Antibody/pm11290804-52-67-73
    Average 93 stars, based on 1 article reviews
    mouse biotinylated anti human il 10r polyclonal ab - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    FIGURE 1. Immunocytochemical staining of intracellular IL-10R in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.

    doi: 10.4049/jimmunol.166.8.5201

    Figure Lengend Snippet: FIGURE 1. Immunocytochemical staining of intracellular IL-10R in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.

    Article Snippet: The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland).

    Techniques: Staining, Negative Control, Control, Light Microscopy

    FIGURE 2. Immunoblotting of IL-10R in human neutrophils. A total cavitas, specific and azurophilic granules, membranes, and cytosol were prepared as described in Materials and Methods. Western blots were probed with a rabbit anti-IL-10R polyclonal Ab. A positive control (mono- nuclear cells) was loaded and blotted in parallel. The molecular masses of protein standards are indicated in kilodaltons.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.

    doi: 10.4049/jimmunol.166.8.5201

    Figure Lengend Snippet: FIGURE 2. Immunoblotting of IL-10R in human neutrophils. A total cavitas, specific and azurophilic granules, membranes, and cytosol were prepared as described in Materials and Methods. Western blots were probed with a rabbit anti-IL-10R polyclonal Ab. A positive control (mono- nuclear cells) was loaded and blotted in parallel. The molecular masses of protein standards are indicated in kilodaltons.

    Article Snippet: The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland).

    Techniques: Western Blot, Positive Control

    FIGURE 3. Translocation of IL-10R from specific granules to the plasma membrane in PMA-stimulated PMN. PMN were incubated for 10 min in the presence or absence of PMA (100 ng/ml) at 37°C. Specific granules and membranes were prepared as described in Materials and Methods. West- ern blots were probed with a rabbit anti-IL-10R polyclonal Ab.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.

    doi: 10.4049/jimmunol.166.8.5201

    Figure Lengend Snippet: FIGURE 3. Translocation of IL-10R from specific granules to the plasma membrane in PMA-stimulated PMN. PMN were incubated for 10 min in the presence or absence of PMA (100 ng/ml) at 37°C. Specific granules and membranes were prepared as described in Materials and Methods. West- ern blots were probed with a rabbit anti-IL-10R polyclonal Ab.

    Article Snippet: The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and mouse biotinylated anti-human IL-10R polyclonal Ab (R&D Systems, Abingdon, U.K.); rat anti-human IL-10R mAb (3F9) (a gift from Kevin Moore, DNAX Research Institute, Palo Alto, CA); rabbit anti-human IL-10R polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA); peroxidase-conjugated swine anti-goat Ig (BioSource International, Camarillo, CA); Dako LSAB alkaline phosphatase kit (Dakopatts, Glostrup, Denmark); purified monoclonal mouse anti-human CD11b; FITC-streptavidin (Immunotech, Marseille, France); FCS (Life Technologies Laboratories, Grand Island, NY), and hydroethidine (HE; Fluka, Buchs, Switzerland).

    Techniques: Translocation Assay, Clinical Proteomics, Membrane, Incubation