mouse biotinylated anti human il 10r polyclonal ab (R&D Systems)
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Mouse Biotinylated Anti Human Il 10r Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 67 article reviews
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1) Product Images from "Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators."
Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
Journal: Journal of immunology (Baltimore, Md. : 1950)
doi: 10.4049/jimmunol.166.8.5201
Figure Legend Snippet: FIGURE 1. Immunocytochemical staining of intracellular IL-10R in whole blood smears. A, Negative control: no staining with control Ig. B, Intracellular fuchsia staining was observed with the specific monoclonal anti-IL-10R Ab. Smears were examined by light microscopy at 31200.
Techniques Used: Staining, Negative Control, Control, Light Microscopy
Figure Legend Snippet: FIGURE 2. Immunoblotting of IL-10R in human neutrophils. A total cavitas, specific and azurophilic granules, membranes, and cytosol were prepared as described in Materials and Methods. Western blots were probed with a rabbit anti-IL-10R polyclonal Ab. A positive control (mono- nuclear cells) was loaded and blotted in parallel. The molecular masses of protein standards are indicated in kilodaltons.
Techniques Used: Western Blot, Positive Control
Figure Legend Snippet: FIGURE 3. Translocation of IL-10R from specific granules to the plasma membrane in PMA-stimulated PMN. PMN were incubated for 10 min in the presence or absence of PMA (100 ng/ml) at 37°C. Specific granules and membranes were prepared as described in Materials and Methods. West- ern blots were probed with a rabbit anti-IL-10R polyclonal Ab.
Techniques Used: Translocation Assay, Clinical Proteomics, Membrane, Incubation
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Recombinant:Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators. Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and Produced:Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators. Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and Protease Inhibitor:Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators. Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and Purification:Article Title: Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators. Article Snippet: .. The reagents and sources were as follows: recombinant human TNF-a (rhTNF-a; 105 U/ml), IL-1b (105 U/ml), and IL-8 (77 aa) produced by endothelial cells (Genzyme, Cambridge, MA); GM-CSF (1.2 3 105 ng/ml; Schering-Plough, Kenilworth, NJ); LPS endotoxin from Escherichia coli (O55:B5), PMA, diisopropylfluorophosphate (DFP), fMLP, primaquine, and a protease inhibitor mixture (Sigma, St. Louis, MO); pentoxifylline (PTX; Hoechst, Paris-La-Défense, France); rhIL-10 (5 mg/ml), biotinylated rhIL-10, FITC-avidin, and |